Article
Improved genetic selection for screening bacteriophage libraries by homologous recombination in vivo.
Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1990
Kurnit D M, Seed B
Abstract excerpt
Three major difficulties have hindered the general application of in vivo recombination techniques to library screening: (i) the original selection could not be applied to libraries prepared in phage vectors lacking amber mutations, (ii) nonirradiated packaging extracts gave high backgrounds even when amber mutated vectors were used, and (iii) most red- vectors lacked rap, a recently discovered phage gene...
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