Article
Improved genetic selection for screening bacteriophage libraries by homologous recombination in vivo.
Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1990
Kurnit D M, Seed B
Abstract excerpt
Three major difficulties have hindered the general application of in vivo recombination techniques to library screening: (i) the original selection could not be applied to libraries prepared in phage vectors lacking amber mutations, (ii) nonirradiated packaging extracts gave high backgrounds even...
Topics
- Base Sequence
- Coliphages
- DNA, Viral
- Escherichia coli
- Gene Library
- Genetic Techniques
- Genotype
- Plasmids
- Recombination, Genetic
- Restriction Mapping