Recombination-promoting activity of the bacteriophage lambda Rap protein in Escherichia coli K-12.

doi:10.1128/JB.184.16.4626-4629.2002

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Article

Recombination-promoting activity of the bacteriophage lambda Rap protein in Escherichia coli K-12.

Journal of bacteriology - 1 Aug 2002

Poteete Anthony R, Fenton Anita C, Wang Hsinju R

Abstract excerpt

The rap gene of bacteriophage lambda was placed in the chromosome of an Escherichia coli K-12 strain in which the recBCD gene cluster had previously been replaced by the lambda red genes and in which the recG gene had been deleted. Recombination between linear double-stranded DNA molecules and the chromosome was tested in variants of the recGDelta red(+) rap(+) strain bearing mutations in genes known to affect...

Topics

Bacterial Proteins
Bacteriophage lambda
Chromosomes, Bacterial
Endodeoxyribonucleases
Escherichia coli
Escherichia coli Proteins
Phenotype
Recombination, Genetic

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Identifiers

PMID: 12142434
DOI: 10.1128/JB.184.16.4626-4629.2002
PMCID: PMC135263

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