Article
Construction of Escherichia coli K12 phr deletion and insertion mutants by gene replacement.
Mutation research - 1 Jan 1991
Akasaka S, Yamamoto K
Abstract excerpt
We replaced an Escherichia coli phr gene by a 1.4-kb fragment of DNA coding for resistance to chloramphenicol. Characterization of 2 deletions (phr-19 and phr-36) and 1 insertion (phr-34) in the phr gene revealed no photoreactivation. Photoreactivation-deficient strains of either recA56 or lexA1(ind-) were more sensitive to UV radiation in the dark than phr-proficient counterparts. The presence of the phr defect...
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