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Article

Construction of Escherichia coli K12 phr deletion and insertion mutants by gene replacement.

Mutation research - 1 Jan 1991

Akasaka S, Yamamoto K

Abstract excerpt

We replaced an Escherichia coli phr gene by a 1.4-kb fragment of DNA coding for resistance to chloramphenicol. Characterization of 2 deletions (phr-19 and phr-36) and 1 insertion (phr-34) in the phr gene revealed no photoreactivation. Photoreactivation-deficient strains of either recA56 or lexA1(ind-) were more sensitive to UV radiation in the dark than phr-proficient counterparts. The presence of the phr defect...

Topics

Alleles
Chloramphenicol O-Acetyltransferase
Chloramphenicol Resistance
Chromosome Deletion
Chromosomes, Bacterial
Cloning, Molecular
DNA Repair
Deoxyribodipyrimidine Photo-Lyase
Escherichia coli
Genes, Bacterial
Genes, Suppressor

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Light

Mutagenesis, Insertional

Restriction Mapping

Ultraviolet Rays

Source

Identifiers

PMID: 1986272
DOI: 10.1016/0921-8777(91)90037-p

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1986272-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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