Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

RNA polymerase II subunit composition, stoichiometry, and phosphorylation.

Molecular and cellular biology - 1 May 1990

Kolodziej P A, Woychik N, Liao S M, Young R A

Abstract excerpt

RNA polymerase II subunit composition, stoichiometry, and phosphorylation were investigated in Saccharomyces cerevisiae by attaching an epitope coding sequence to a well-characterized RNA polymerase II subunit gene (RPB3) and by immunoprecipitating the product of this gene with its associated polypeptides. The immunopurified enzyme catalyzed alpha-amanitin-sensitive RNA synthesis in vitro. The 10 polypeptides...

Topics

Cell Cycle
Cloning, Molecular
Macromolecular Substances
Molecular Weight
Mutation
Phosphorylation
Precipitin Tests
RNA Polymerase II
Recombinant Proteins
Saccharomyces cerevisiae

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Open in PubMed Open full text

Identifiers

PMID: 2183013
DOI: 10.1128/mcb.10.5.1915-1920.1990
PMCID: PMC360537

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2183013-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

RNA polymerase II subunit composition, stoichiometry, and phosphorylation.PMID 2183013Read ↗

Rpb3, stoichiometry and sequence determinants of the assembly into yeast RNA polymerase II in vivo.Semantic proximity Read ↗

Gene RPA43 in Saccharomyces cerevisiae encodes an essential subunit of RNA polymerase I.Semantic proximity Read ↗

Mutations in the three largest subunits of yeast RNA polymerase II that affect enzyme assembly.Semantic proximity Read ↗

Site specific phosphorylation of yeast RNA polymerase I.Semantic proximity Read ↗

Loss of the Rpb4/Rpb7 subcomplex in a mutant form of the Rpb6 subunit shared by RNA polymerases I, II, and III.Semantic proximity Read ↗

Diversification of function by different isoforms of conventionally shared RNA polymerase subunits.Semantic proximity Read ↗

Domainal organization of the lower eukaryotic homologs of the yeast RNA polymerase II core subunit Rpb7 reflects functional conservation.Semantic proximity Read ↗

Gpn2 and Rba50 Directly Participate in the Assembly of the Rpb3 Subcomplex in the Biogenesis of RNA Polymerase II.Semantic proximity Read ↗

Unstructured N terminus of the RNA polymerase II subunit Rpb4 contributes to the interaction of Rpb4.Rpb7 subcomplex with the core RNA polymerase II of Saccharomyces cerevisiae.Semantic proximity Read ↗

Distinct regions of RPB11 are required for heterodimerization with RPB3 in human and yeast RNA polymerase II.Semantic proximity Read ↗

Rba50 and Gpn2 recruit the second largest subunits for the assembly of RNA polymerase II and III.Semantic proximity Read ↗

Functional organization of the Rpb5 subunit shared by the three yeast RNA polymerases.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.