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Article

RNA polymerase II subunit composition, stoichiometry, and phosphorylation.

Molecular and cellular biology - 1 May 1990

Kolodziej P A, Woychik N, Liao S M, Young R A

Abstract excerpt

RNA polymerase II subunit composition, stoichiometry, and phosphorylation were investigated in Saccharomyces cerevisiae by attaching an epitope coding sequence to a well-characterized RNA polymerase II subunit gene (RPB3) and by immunoprecipitating the product of this gene with its associated polypeptides. The immunopurified enzyme catalyzed alpha-amanitin-sensitive RNA synthesis in vitro. The 10 polypeptides...

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Identifiers

PMID: 2183013
DOI: 10.1128/mcb.10.5.1915-1920.1990
PMCID: PMC360537

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