Article
Site specific phosphorylation of yeast RNA polymerase I.
Nucleic acids research - 1 Feb 2008
Gerber Jochen, Reiter Alarich, Steinbauer Robert, Jakob Steffen, Kuhn Claus-Dieter, Cramer Patrick, Griesenbeck Joachim, Milkereit Philipp, Tschochner Herbert
Abstract excerpt
All nuclear RNA polymerases are phosphoprotein complexes. Yeast RNA polymerase I (Pol I) contains approximately 15 phosphate groups, distributed to 5 of the 14 subunits. Information about the function of the single phosphosites and their position in the primary, secondary and tertiary structure is lacking. We used a rapid and efficient way to purify yeast RNA Pol I to determine 13 phosphoserines and -threonines....
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