Article
Rpb3, stoichiometry and sequence determinants of the assembly into yeast RNA polymerase II in vivo.
The Journal of biological chemistry - 1 May 1998
Svetlov V, Nolan K, Burgess R R
Abstract excerpt
Stoichiometry of the third largest subunit (Rpb3) of the yeast RNA polymerase II is a subject of continuing controversy. In this work we utilized immunoaffinity and nickel-chelate chromatographic techniques to isolate the RNA polymerase II species assembled in vivo in the presence of the His6-tag...
Topics
- Amino Acid Sequence
- Chromatography, Affinity
- Molecular Sequence Data
- Mutation
- Phylogeny
- RNA Polymerase II
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
