Article
Rpb3, stoichiometry and sequence determinants of the assembly into yeast RNA polymerase II in vivo.
The Journal of biological chemistry - 1 May 1998
Svetlov V, Nolan K, Burgess R R
Abstract excerpt
Stoichiometry of the third largest subunit (Rpb3) of the yeast RNA polymerase II is a subject of continuing controversy. In this work we utilized immunoaffinity and nickel-chelate chromatographic techniques to isolate the RNA polymerase II species assembled in vivo in the presence of the His6-tagged and untagged Rpb3. The distribution pattern of tagged and untagged subunits among the RNA polymerase II molecules...
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