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Article

Generation of a cell culture-adapted hepatitis C virus with longer half life at physiological temperature.

PloS one - 1 Jan 2011

Kim Chon Saeng, Keum Sun Ju, Jang Sung Key

Abstract excerpt

BACKGROUND: We previously reported infectious HCV clones that contain the convenient reporters, green fluorescent protein (GFP) and Renilla luciferase (Rluc), in the NS5a-coding sequence. Although these viruses were useful in monitoring viral proliferation and screening of anti-HCV drugs, the infectivity and yield of the viruses were low. METHODOLOGY/PRINCIPAL FINDINGS: In order to obtain a highly efficient HCV...

Topics

Base Sequence
Cell Line
DNA Primers
Half-Life
Hepacivirus
Humans
Mutation
Polymerase Chain Reaction
Temperature
Viral Proteins
Virulence

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PMID: 21829654
DOI: 10.1371/journal.pone.0022808
PMCID: PMC3150383

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