Article
Development and application of hepatitis C reporter viruses with genotype 1 to 7 core-nonstructural protein 2 (NS2) expressing fluorescent proteins or luciferase in modified JFH1 NS5A.
Journal of virology - 1 Sept 2011
Gottwein Judith M, Jensen Tanja B, Mathiesen Christian K, Meuleman Philip, Serre Stephanie B N, Lademann Jacob B, Ghanem Lubna, Scheel Troels K H, Leroux-Roels Geert, Bukh Jens
Abstract excerpt
To facilitate genotype-specific high-throughput studies of hepatitis C virus (HCV), we have developed reporter viruses using JFH1-based recombinants expressing core-nonstructural protein 2 (NS2) of genotype 1 to 7 prototype isolates. We introduced enhanced green fluorescent protein (EGFP) into NS5A domain III of the genotype 2a virus J6/JFH1 [2a(J6)]. During Huh7.5 cell culture adaptation, 2a(J6)-EGFP acquired a...
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