Article
Permissivity of primary human hepatocytes and different hepatoma cell lines to cell culture adapted hepatitis C virus.
PloS one - 1 Jan 2013
Helle Francois, Brochot Etienne, Fournier Carole, Descamps Véronique, Izquierdo Laure, Hoffmann Thomas W, Morel Virginie, Herpe Yves-Edouard, Bengrine Abderrahmane, Belouzard Sandrine, Wychowski Czeslaw, Dubuisson Jean, Francois Catherine, Regimbeau Jean-Marc, Castelain Sandrine, Duverlie Gilles
Abstract excerpt
Significant progress has been made in Hepatitis C virus (HCV) culture since the JFH1 strain cloning. However, developing efficient and physiologically relevant culture systems for all viral genotypes remains an important goal. In this work, we aimed at producing a high titer JFH1 derived virus to test different hepatic cells' permissivity. To this end, we performed successive infections and obtained a JFH1...
Topics
- Adaptation, Physiological
- Carcinoma, Hepatocellular
- DNA Mutational Analysis
- Hep G2 Cells
- Hepacivirus
- Host-Pathogen Interactions
- Humans
- Immunity, Innate
- Mutation