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Article

Production of infectious chimeric hepatitis C virus genotype 2b harboring minimal regions of JFH-1.

Journal of virology - 1 Feb 2012

Murayama Asako, Kato Takanobu, Akazawa Daisuke, Sugiyama Nao, Date Tomoko, Masaki Takahiro, Nakamoto Shingo, Tanaka Yasuhito, Mizokami Masashi, Yokosuka Osamu, Nomoto Akio, Wakita Takaji

Abstract excerpt

To establish a cell culture system for chimeric hepatitis C virus (HCV) genotype 2b, we prepared a chimeric construct harboring the 5' untranslated region (UTR) to the E2 region of the MA strain (genotype 2b) and the region of p7 to the 3' UTR of the JFH-1 strain (genotype 2a). This chimeric RNA (MA/JFH-1.1) replicated and produced infectious virus in Huh7.5.1 cells. Replacement of the 5' UTR of this chimera with...

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Identifiers

PMID: 22156532
DOI: 10.1128/JVI.05386-11
PMCID: PMC3302416

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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