Article
Analysis of GPT activity in mammalian cells with a chromosomally integrated shuttle vector containing altered gpt genes.
Somatic cell and molecular genetics - 1 Mar 1990
Gelbert L M, Wilson M M, Davidson R L
Abstract excerpt
The molecular mechanisms of reversion in mammalian cells were studied utilizing the pZipGptNeo shuttle vector, with the bacterial gpt gene in the vector integrated into the chromosomal DNA of mouse cells. From mutant cell lines containing gpt genes with single base changes, revertants were selected for the reappearance of GPT activity. The copy number and expression of the gpt genes in such revertants were...
Topics
- Animals
- Base Sequence
- Cell Line
- Chromosomes
- Colony Count, Microbial
- DNA
- Escherichia coli
- Gene Amplification
- Gene Expression
- Genes, Bacterial
- Genetic Vectors