Silencing of mouse Aprt is a gradual process in differentiated cells.

doi:10.1128/MCB.23.13.4461-4470.2003

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Article

Silencing of mouse Aprt is a gradual process in differentiated cells.

Molecular and cellular biology - 1 Jul 2003

Yates Phillip A, Burman Robert, Simpson James, Ponomoreva Olga N, Thayer Mathew J, Turker Mitchell S

Abstract excerpt

Mouse Aprt constructs that are highly susceptible to DNA methylation-associated inactivation in embryonal carcinoma cells were transfected into differentiated cells, where they were expressed. Construct silencing was induced by either whole-cell fusion of the expressing differentiated cells with embryonal carcinoma cells or by treatment of the differentiated cells with the DNA demethylating agent...

Topics

Adenine Phosphoribosyltransferase
Alleles
Animals
Azacitidine
Binding Sites
Blotting, Southern
Cell Differentiation
Cell Line
Cloning, Molecular
CpG Islands
DNA Methylation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Decitabine

Gene Silencing

In Situ Hybridization, Fluorescence

Mice

Models, Genetic

Phenotype

Reverse Transcriptase Polymerase Chain Reaction

Source

Open in PubMed Open full text

Identifiers

PMID: 12808089
DOI: 10.1128/MCB.23.13.4461-4470.2003
PMCID: PMC164859

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