Article
Thermal energy suppresses mutational defects in DNA unwinding at a yeast replication origin.
Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1990
Umek R M, Kowalski D
Abstract excerpt
Yeast replication origins contain a DNA sequence element whose biological activity correlates with hypersensitivity to single-strand-specific nucleases in negatively supercoiled plasmids. By using two-dimensional gel electrophoresis of plasmid topoisomers, we demonstrate that thermodynamically stable origin unwinding accounts for the nuclease hypersensitivity and, furthermore, that increased thermal energy...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.