Thermal energy suppresses mutational defects in DNA unwinding at a yeast replication origin.

doi:10.1073/pnas.87.7.2486

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Article

Thermal energy suppresses mutational defects in DNA unwinding at a yeast replication origin.

Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1990

Umek R M, Kowalski D

Abstract excerpt

Yeast replication origins contain a DNA sequence element whose biological activity correlates with hypersensitivity to single-strand-specific nucleases in negatively supercoiled plasmids. By using two-dimensional gel electrophoresis of plasmid topoisomers, we demonstrate that thermodynamically st...

Topics

Base Sequence
Cloning, Molecular
DNA Replication
DNA, Fungal
Escherichia coli
Molecular Sequence Data
Mutation
Plasmids
Saccharomyces cerevisiae
Thermodynamics

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