Article
Distinguishing the roles of Topoisomerases I and II in relief of transcription-induced torsional stress in yeast rRNA genes.
Molecular and cellular biology - 1 Feb 2011
French Sarah L, Sikes Martha L, Hontz Robert D, Osheim Yvonne N, Lambert Tashima E, El Hage Aziz, Smith Mitchell M, Tollervey David, Smith Jeffrey S, Beyer Ann L
Abstract excerpt
To better understand the role of topoisomerase activity in relieving transcription-induced supercoiling, yeast genes encoding rRNA were visualized in cells deficient for either or both of the two major topoisomerases. In the absence of both topoisomerase I (Top1) and topoisomerase II (Top2) activity, processivity was severely impaired and polymerases were unable to transcribe through the 6.7-kb gene. Loss of Top1...
Topics
- DNA Polymerase I
- DNA Replication
- DNA Topoisomerases, Type I
- DNA Topoisomerases, Type II
- Enzyme Activation
- Genes, Fungal
- Genes, rRNA
- Models, Biological
- Mutation
- Nucleic Acid Conformation
- Nucleic Acid Denaturation
- Ribonuclease H