Uncoupling gene activity from chromatin structure: promoter mutations can inactivate transcription of the yeast HSP82 gene without eliminating nucleosome-free regions.

doi:10.1073/pnas.89.19.9166

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Uncoupling gene activity from chromatin structure: promoter mutations can inactivate transcription of the yeast HSP82 gene without eliminating nucleosome-free regions.

Proceedings of the National Academy of Sciences of the United States of America - 1 Oct 1992

Lee M S, Garrard W T

Abstract excerpt

DNase I-hypersensitive sites represent "nucleosome-free" regions in chromatin where the underlying DNA sequence is highly accessible to trans-acting proteins. Here we demonstrate that it is possible to uncouple gene activity from hypersensitive site formation. Point or substitution mutations were introduced into the promoter of the yeast chromosomal HSP82 gene, encoding the 83-kDa heat shock protein (HSP), via...

Topics

Alleles
Base Sequence
Binding Sites
DNA, Fungal
Deoxyribonuclease I
Gene Expression Regulation, Fungal
Genes, Fungal
Heat-Shock Proteins
Hot Temperature
Kinetics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutagenesis, Site-Directed

Oligodeoxyribonucleotides

Promoter Regions, Genetic

RNA, Messenger

Saccharomyces cerevisiae

TATA Box

Transcription, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 1409619
DOI: 10.1073/pnas.89.19.9166
PMCID: PMC50086

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1409619-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Uncoupling gene activity from chromatin structure: promoter mutations can inactivate transcription of the yeast HSP82 gene without eliminating nucleosome-free regions.PMID 1409619Read ↗

A critical role for heat shock transcription factor in establishing a nucleosome-free region over the TATA-initiation site of the yeast HSP82 heat shock gene.Semantic proximity Read ↗

SIR repression of a yeast heat shock gene: UAS and TATA footprints persist within heterochromatin.Semantic proximity Read ↗

Heat shock factor gains access to the yeast HSC82 promoter independently of other sequence-specific factors and antagonizes nucleosomal repression of basal and induced transcription.Semantic proximity Read ↗

SAGA and Rpd3 chromatin modification complexes dynamically regulate heat shock gene structure and expression.Semantic proximity Read ↗

Remarkable site specificity of local transposition into the Hsp70 promoter of Drosophila melanogaster.Semantic proximity Read ↗

Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in Saccharomyces cerevisiae.Semantic proximity Read ↗

Conditional silencing: the HMRE mating-type silencer exerts a rapidly reversible position effect on the yeast HSP82 heat shock gene.Semantic proximity Read ↗

Cell cycle-dependent binding of yeast heat shock factor to nucleosomes.Semantic proximity Read ↗

Transcription-coupled DNA repair in yeast transcription factor IIE (TFIIE) mutants.Semantic proximity Read ↗

Temperature-sensitive mutants of hsp82 of the budding yeast Saccharomyces cerevisiae.Semantic proximity Read ↗

The molecular chaperone Hsp90 mediates heme activation of the yeast transcriptional activator Hap1.Semantic proximity Read ↗

DNA sequence requirements for generating paused polymerase at the start of hsp70.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.