Article
Drosophila nonsense suppressors: functional analysis in Saccharomyces cerevisiae, Drosophila tissue culture cells and Drosophila melanogaster.
Genetics - 1 Nov 1990
Garza D, Medhora M M, Hartl D L
Abstract excerpt
Amber (UAG) and opal (UGA) nonsense suppressors were constructed by oligonucleotide site-directed mutagenesis of two Drosophila melanogaster leucine-tRNA genes and tested in yeast, Drosophila tissue culture cells and transformed flies. Suppression of a variety of amber and opal alleles occurs in yeast. In Drosophila tissue culture cells, the mutant tRNAs suppress hsp70:Adh (alcohol dehydrogenase) amber and opal...
Topics
- Alcohol Dehydrogenase
- Animals
- Base Sequence
- Blotting, Western
- Cells, Cultured
- DNA Transposable Elements
- Drosophila melanogaster
- Molecular Sequence Data
- Mutation
- RNA, Transfer, Leu
- Reproduction