Article
Construction of an opal suppressor by oligonucleotide-directed mutagenesis of a Saccharomyces cerevisiae tRNA(Trp) gene.
Molecular and cellular biology - 1 Aug 1990
Atkin A L, Roy K L, Bell J B
Abstract excerpt
In vitro mutagenesis was used to create putative opal suppressor alleles of a tRNA(Trp) gene of Saccharomyces cerevisiae. The construct with the requisite anticodon change did not result in an active suppressor, whereas when a second change was introduced into the portion of the gene encoding the...
Topics
- Alleles
- Base Sequence
- Cloning, Molecular
- DNA, Fungal
- Genes, Fungal
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Oligonucleotide Probes
- Plasmids
- RNA, Transfer, Amino Acid-Specific
- RNA, Transfer, Trp
- Restriction Mapping
- Suppression, Genetic
- Transcription, Genetic