Article
Nonsense suppression of the major rhodopsin gene of Drosophila.
Genetics - 1 Mar 1992
Washburn T, O'Tousa J E
Abstract excerpt
We placed UAA, UAG and UGA nonsense mutations at two leucine codons, Leu205 and Leu309, in Drosophila's major rhodopsin gene, ninaE, by site-directed mutagenesis, and then created the corresponding mutants by P element-mediated transformation of a ninaE deficiency strain. In the absence of a genetic suppressor, flies harboring any of the nonsense mutations at the 309 site, but not the 205 site, show increased...
Topics
- Alleles
- Amino Acid Sequence
- Animals
- Base Sequence
- DNA
- Drosophila
- Electrophysiology
- Microscopy, Electron
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Photoreceptor Cells