Article
Genetic and molecular analysis of eight tRNA(Trp) amber suppressors in Caenorhabditis elegans.
Journal of molecular biology - 5 Sept 1990
Kondo K, Makovec B, Waterston R H, Hodgkin J
Abstract excerpt
Over 100 revertants of five different amber mutants were analyzed by Southern blot hybridization using synthetic oligomers as probes to detect a single base change at the anticodon, CCA to CTA (amber), of tRNA(Trp) genes of Caenohrabditis elegans. Of the 12 members of the tRNA(Trp) gene family, a...
Topics
- Animals
- Base Sequence
- Caenorhabditis
- Chromosome Mapping
- Gene Expression Regulation
- Genetic Linkage
- Molecular Sequence Data
- Multigene Family
- Mutation
- Oligonucleotide Probes
- Polymerase Chain Reaction
- RNA, Transfer, Amino Acid-Specific
- RNA, Transfer, Trp
- Suppression, Genetic