Article
Plant nonsense suppressor tRNA(Tyr) genes are expressed at very low levels in vitro due to inefficient splicing of the intron-containing pre-tRNAs.
Nucleic acids research - 25 Feb 1991
Szweykowska-Kulinska Z, Beier H
Abstract excerpt
Oligonucleotide-directed mutagenesis was used to generate amber, ochre and opal suppressors from cloned Arabidopsis and Nicotiana tRNA(Tyr) genes. The nonsense suppressor tRNA(Tyr) genes were efficiently transcribed in HeLa and yeast nuclear extracts, however, intron excision from all mutant pre-tRNAs(Tyr) was severely impaired in the homologous wheat germ extract as well as in the yeast in vitro splicing system....
Topics
- Base Sequence
- Electrophoresis, Polyacrylamide Gel
- Genes, Suppressor
- Introns
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Plants
- RNA Precursors
- RNA Splicing
- RNA, Transfer, Tyr