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Article

Variant identification in multi-sample pools by illumina genome analyzer sequencing.

Journal of biomolecular techniques : JBT - 1 Jul 2011

Margraf Rebecca L, Durtschi Jacob D, Dames Shale, Pattison David C, Stephens Jack E, Voelkerding Karl V

Abstract excerpt

Multi-sample pooling and Illumina Genome Analyzer (GA) sequencing allows high throughput sequencing of multiple samples to determine population sequence variation. A preliminary experiment, using the RET proto-oncogene as a model, predicted ≤ 30 samples could be pooled to reliably detect singleton variants without requiring additional confirmation testing. This report used 30 and 50 sample pools to test the...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 21738440
PMCID: PMC3121147

Genes

RET

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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