Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Proof of concept for multiplex amplicon sequencing for mutation identification using the MinION nanopore sequencer.

Scientific reports - 20 May 2022

Whitford Whitney, Hawkins Victoria, Moodley Kriebashne S, Grant Matthew J, Lehnert Klaus, Snell Russell G, Jacobsen Jessie C

Abstract excerpt

Rapid, cost-effective identification of genetic variants in small candidate genomic regions remains a challenge, particularly for less well equipped or lower throughput laboratories. The application of Oxford Nanopore Technologies' MinION sequencer has the potential to fulfil this requirement. We demonstrate a proof of concept for a multiplexing assay that pools PCR amplicons for MinION sequencing to enable...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Genomics
Share to High-Throughput Nucleotide Sequencing

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Mutation

Share to Nanopore Sequencing

Share to Sequence Analysis, DNA

Source

Open in PubMed Open full text

Identifiers

PMID: 35595858
DOI: 10.1038/s41598-022-12613-7
PMCID: PMC9122479

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Proof of concept for multiplex amplicon sequencing for mutation identification using the MinION nanopore sequencer.PMID 35595858Read ↗

Optimised multiplex amplicon sequencing for mutation identification using the MinION nanopore sequencerSemantic proximity Read ↗

A Multiplex Pharmacogenetics Assay using the MinION Nanopore Sequencing DeviceSemantic proximity Read ↗

Evaluation of the detection of GBA missense mutations and other variants using the Oxford Nanopore MinIONSemantic proximity Read ↗

Flexible, scalable, and efficient targeted resequencing on a benchtop sequencer for variant detection in clinical practice.Semantic proximity Read ↗

Detection of <i>GBA</i> missense mutations and other variants using the Oxford Nanopore MinIONSemantic proximity Read ↗

MinION rapid sequencing: Review of potential applications in neurosurgerySemantic proximity Read ↗

Optimization of a multiplex minisequencing protocol for population studies and medical genetics.Semantic proximity Read ↗

Multisite Evaluation of an Amplification-based Nanopore Sequencing Solution to Analyze Challenging Clinically Relevant Variants in Genes Associated with Hereditary DiseasesSemantic proximity Read ↗

Phasing of de novo mutations using a scaled-up multiple amplicon long-read sequencing approachSemantic proximity Read ↗

Nanopore sequencing and assembly of a human genome with ultra-long readsSemantic proximity Read ↗

Phasing of de novo mutations using a scaled-up multiple amplicon long-read sequencing approach.Semantic proximity Read ↗

ECNano: A Cost-Effective Workflow for Target Enrichment Sequencing and Accurate Variant Calling on 4,800 Clinically Significant Genes Using a Single MinION FlowcellSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.