Article

Optimised multiplex amplicon sequencing for mutation identification using the MinION nanopore sequencer

2021-09-24

Abstract excerpt

<h4>Objective</h4> Rapid, cost-effective identification of genetic variants in small candiate genomic regions remains a challenge, particularly for less well equipped or lower throughput laboratories. Application of Oxford Nanopore Technologies’ MinION sequencer has the potential to fulfil this requirement. We have developed a multiplexing assay which pools PCR amplicons for MinION sequencing to enable sequencing...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 8d0c6975-5e76-59cb-b876-d322788a7014
DOI: 10.1101/2021.09.21.461312

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Optimised multiplex amplicon sequencing for mutation identification using the MinION nanopore sequencerDOI 10.1101/2021.09.21.461312Read

Proof of concept for multiplex amplicon sequencing for mutation identification using the MinION nanopore sequencer.Semantic proximity Read

A Multiplex Pharmacogenetics Assay using the MinION Nanopore Sequencing DeviceSemantic proximity Read

Detection of <i>GBA</i> missense mutations and other variants using the Oxford Nanopore MinIONSemantic proximity Read

Evaluation of the detection of GBA missense mutations and other variants using the Oxford Nanopore MinIONSemantic proximity Read

Multisite Evaluation of an Amplification-based Nanopore Sequencing Solution to Analyze Challenging Clinically Relevant Variants in Genes Associated with Hereditary DiseasesSemantic proximity Read

MinION rapid sequencing: Review of potential applications in neurosurgerySemantic proximity Read

ECNano: A Cost-Effective Workflow for Target Enrichment Sequencing and Accurate Variant Calling on 4,800 Clinically Significant Genes Using a Single MinION FlowcellSemantic proximity Read

Flexible, scalable, and efficient targeted resequencing on a benchtop sequencer for variant detection in clinical practice.Semantic proximity Read

Nanopore sequencing and assembly of a human genome with ultra-long readsSemantic proximity Read

Optimization of a multiplex minisequencing protocol for population studies and medical genetics.Semantic proximity Read

Nanopore Third-Generation Sequencing for Comprehensive Analysis of Hemoglobinopathy VariantsSemantic proximity Read

Assessing the readiness of Oxford Nanopore sequencing for clinical genomics applicationsSemantic proximity Read