Article
Accurate variant detection across non-amplified and whole genome amplified DNA using targeted next generation sequencing.
BMC genomics - 20 Sept 2012
ElSharawy Abdou, Warner Jason, Olson Jeff, Forster Michael, Schilhabel Markus B, Link Darren R, Rose-John Stefan, Schreiber Stefan, Rosenstiel Philip, Brayer James, Franke Andre
Abstract excerpt
BACKGROUND: Many hypothesis-driven genetic studies require the ability to comprehensively and efficiently target specific regions of the genome to detect sequence variations. Often, sample availability is limited requiring the use of whole genome amplification (WGA). We evaluated a high-throughput microdroplet-based PCR approach in combination with next generation sequencing (NGS) to target 384 discrete exons...
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