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Article

Multi-sample pooling and illumina genome analyzer sequencing methods to determine gene sequence variation for database development.

Journal of biomolecular techniques : JBT - 1 Sept 2010

Margraf Rebecca L, Durtschi Jacob D, Dames Shale, Pattison David C, Stephens Jack E, Mao Rong, Voelkerding Karl V

Abstract excerpt

Determination of sequence variation within a genetic locus to develop clinically relevant databases is critical for molecular assay design and clinical test interpretation, so multisample pooling for Illumina genome analyzer (GA) sequencing was investigated using the RET proto-oncogene as a model. Samples were Sanger-sequenced for RET exons 10, 11, and 13-16. Ten samples with 13 known unique variants ("singleton...

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Identifiers

PMID: 20808642
PMCID: PMC2922832

Genes

RET

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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