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Article

Kinetic studies of cAMP-induced allosteric changes in mutants T127I, S128A, and T127I/S128A of the cAMP receptor protein from Escherichia coli.

The Journal of biological chemistry - 31 Oct 2003

Polit Agnieszka, Bonarek Piotr, Kepys Barbara, Kedracka-Krok Sylwia, Górecki Andrzej, Wasylewski Zygmunt

Abstract excerpt

The cAMP receptor protein (CRP) regulates the expression of several genes in Escherichia coli. The protein is a homodimer, and each monomer is folded into two distinct structural domains. After allosteric transitions resulting from the binding of cAMP, CRP specifically binds to DNA and activates transcription. We have used stopped-flow fluorometry measurements of CRP mutants bearing amino acid substitutions...

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PMID: 12939272
DOI: 10.1074/jbc.M306398200

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CRP

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Kinetic studies of cAMP-induced allosteric changes in mutants T127I, S128A, and T127I/S128A of the cAMP receptor protein from Escherichia coli.PMID 12939272Read ↗

Study of highly constitutively active mutants suggests how cAMP activates cAMP receptor protein.Semantic proximity Read ↗

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The effect of Ser 128 substitution on the structure and stability of cAMP receptor protein from Escherichia coli.Semantic proximity Read ↗

Effect of mutations at the monomer-monomer interface of cAMP receptor protein on specific DNA binding.Semantic proximity Read ↗

Allosteric regulation of the cAMP receptor protein.Semantic proximity Read ↗

The N-terminal capping propensities of the D-helix modulate the allosteric activation of the Escherichia coli cAMP receptor protein.Semantic proximity Read ↗

Intersubunit association induces unique allosteric dependence of the T127L CRP mutant on pH.Semantic proximity Read ↗

Characterization of the binding of cAMP and cGMP to the CRP*598 mutant of the E. coli cAMP receptor protein.Semantic proximity Read ↗

Entropic nature of the interaction between promoter bound CRP mutants and RNA polymerase.Semantic proximity Read ↗

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Position 127 amino acid substitutions affect the formation of CRP:cAMP:lacP complexes but not CRP:cAMP:RNA polymerase complexes at lacP.Semantic proximity Read ↗

Determination of the conformations of cAMP receptor protein and its T127L,S128A mutant with and without cAMP from small angle neutron scattering measurements.Semantic proximity Read ↗

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