Article
Evaluation of the effects of 18 non-synonymous single-nucleotide polymorphisms of CYP450 2C19 on in vitro drug inhibition potential by a fluorescence-based high-throughput assay.
Xenobiotica; the fate of foreign compounds in biological systems - 1 Sept 2011
Wang Huijuan, Kim Raymond A, Sun Dongxu, Gao Yiwen, Wang Hao, Zhu Juanli, Chen Chao
Abstract excerpt
To comprehensively understand the effects of CYP2C19 genetic polymorphisms on inhibition-based drug-drug interactions (DDIs), 18 human CYP2C19 non-synonymous single-nucleotide polymorphic variants and the wild-type isoform (CYP2C19.1A) were expressed in yeast cells. Using a fluorescence-based high-throughput method, the kinetic constants of these variants, as well as the inhibition constants for 10 drugs, were...
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