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Article

Linker insertion mutagenesis of herpesviruses: inactivation of single genes within the Us region of pseudorabies virus.

Journal of virology - 1 Oct 1990

de Wind N, Zijderveld A, Glazenburg K, Gielkens A, Berns A

Abstract excerpt

We describe a technique for the systematic inactivation of nonessential genes within the genome of a herpesvirus without the requirement for phenotypic selection. This technique is based on the insertion of an oligonucleotide containing translational stop codons at a random site within a large cloned viral DNA fragment. Mutant virus is then reconstituted by cotransfection with overlapping viral clones, together...

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Identifiers

PMID: 2168958
DOI: 10.1128/JVI.64.10.4691-4696.1990
PMCID: PMC247954

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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