Article
Linker insertion mutagenesis of herpesviruses: inactivation of single genes within the Us region of pseudorabies virus.
Journal of virology - 1 Oct 1990
de Wind N, Zijderveld A, Glazenburg K, Gielkens A, Berns A
Abstract excerpt
We describe a technique for the systematic inactivation of nonessential genes within the genome of a herpesvirus without the requirement for phenotypic selection. This technique is based on the insertion of an oligonucleotide containing translational stop codons at a random site within a large cl...
Topics
- Cell Line
- Cell Transformation, Viral
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Viral
- Genes, Viral
- Herpesviridae
- Herpesvirus 1, Suid
- Humans
- Mutation
- Protein Biosynthesis
- Restriction Mapping
- Transcription, Genetic
- Transfection