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Cloning of herpesviral genomes as bacterial artificial chromosomes

28 Feb 2003

Abstract excerpt

Herpesviruses, which are important pathogens for both animals and humans, have large and complex genomes with a coding capacity for up to 225 open reading frames (ORFs). Due to the large genome size and the slow replication kinetics in vitro of some herpesviruses, mutagenesis of viral genes in the context of the viral genome by conventional recombination methods in cell culture has been difficult. Given that...

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PMID: 12627394
DOI: 10.1002/rmv.380

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