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A simple method for rapid cloning of complete herpesvirus genomes

23 Jan 2024

Abstract excerpt

Herpesviruses are large DNA viruses and include important human and veterinary pathogens. Their genomes can be cloned as bacterial artificial chromosomes (BACs) and genetically engineered in Escherichia coli using BAC recombineering methods. While the recombineering methods are efficient, the initial BAC-cloning step remains laborious. To overcome this limitation, we have developed a simple, rapid, and efficient...

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PMID: 38266652
DOI: 10.1016/j.crmeth.2024.100696

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