Article
Generation and characterization of UL41 null pseudorabies virus variant in vitro and in vivo.
Virology journal - 2 Aug 2018
Ye Chao, Chen Jing, Wang Tao, Xu Jingjing, Zheng Hao, Wu Jiqiang, Li Guoxin, Yu Zhiqing, Tong Wu, Cheng Xuefei, Zhou Shasha, Tong Guangzhi
Abstract excerpt
BACKGROUND: The alphaherpesvirus virion host shutoff (vhs) gene, UL41, can induce degradation of host mRNAs and shut off host protein synthesis. The roles of vhs in HSV-1 and HSV-2 have been studied extensively in previous studies, however, relatively little is known about the vhs protein of PRV. METHODS: A novel method combining CRISPR/Cas9 and Gibson assembly was developed to generate UL41 null PRV variant. The...
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