Article
A role for the poly(A)-binding protein Pab1p in PUF protein-mediated repression.
The Journal of biological chemistry - 23 Sept 2011
Chritton Jacqueline J, Wickens Marvin
Abstract excerpt
PUF proteins regulate translation and mRNA stability throughout eukaryotes. Using a cell-free translation assay, we examined the mechanisms of translational repression of PUF proteins in the budding yeast Saccharomyces cerevisiae. We demonstrate that the poly(A)-binding protein Pab1p is required for PUF-mediated translational repression for two distantly related PUF proteins: S. cerevisiae Puf5p and...
Topics
- 3' Untranslated Regions
- Amino Acid Motifs
- Base Sequence
- Caenorhabditis elegans Proteins
- Genes, Reporter
- Models, Biological
- Molecular Sequence Data
- Mutation
- Poly A
- Poly(A)-Binding Proteins
- Protein Binding