Article
Improved V3 genotyping with duplicate PCR amplification for determining HIV-1 tropism.
The Journal of antimicrobial chemotherapy - 1 Sept 2011
Raymond Stéphanie, Recordon-Pinson Patricia, Saliou Adrien, Delobel Pierre, Nicot Florence, Descamps Diane, Marcelin Anne-Geneviève, Flandre Philippe, Calvez Vincent, Masquelier Bernard, Izopet Jacques
Abstract excerpt
OBJECTIVES: To determine whether genotyping of HIV-1 by duplicate PCR amplification of the region encoding the V3 loop is more sensitive than single PCR for detecting CXCR4-using viruses. PATIENTS AND METHODS: The V3 genotypes of the HIV-1 infecting 152 patients enrolled in the multicentre GenoTropism ANRS study were determined by all the participating laboratories using a single PCR and V3 bulk sequencing. In...
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