Article
Genotypic tropism prediction from paired cell and plasma using single and replicate sequences.
AIDS research and human retroviruses - 1 Jul 2014
Coelho Luana Portes Ozório, Ferreira João Leandro de Paula, Cabral Gabriela Bastos, Guimarães Paula Morena de Souza, Brigido Luis Fernando de Macedo
Abstract excerpt
HIV-1 tropism determination is necessary prior to CCR5 antagonist use as antiretroviral therapy. Genotypic prediction of coreceptor use is a practical alternative to phenotypic tests. Cell DNA and plasma RNA-based prediction has shown discordance in many studies. We evaluate paired cell and plasma either as single or replicate V3 sequences to assess prediction comparability. The HIV-1 partial env region was...
Topics
- Base Sequence
- CD4 Antigens
- Genetic Variation
- HIV-1
- Humans
- RNA, Viral
- Receptors, CCR5
- Receptors, CXCR4
- Sequence Analysis, RNA
- Viral Tropism
- env Gene Products, Human Immunodeficiency Virus