Article
A DNA motif related to the cAMP-responsive element and an exon-located activator protein-2 binding site in the human tissue-type plasminogen activator gene promoter cooperate in basal expression and convey activation by phorbol ester and cAMP.
The Journal of biological chemistry - 25 Aug 1990
Medcalf R L, Rüegg M, Schleuning W D
Abstract excerpt
Tissue-type plasminogen activator (t-PA) gene expression is regulated by the tumor-promoting phorbol ester, phorbol-12-myristate 13-acetate (PMA), by cyclic AMP analogues, and the cAMP agonist, forskolin. Based on nuclear "run-on" transcription assays, t-PA expression is modulated by PMA on the l...
Topics
- Amanitins
- Base Sequence
- Binding Sites
- Cell Line
- Cloning, Molecular
- Cyclic AMP
- Cycloheximide
- DNA
- DNA-Binding Proteins
- Deoxyribonuclease I
- Exons
- Gene Expression Regulation, Enzymologic
- Genes
- HeLa Cells
- Humans
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes