Article
Transcriptional induction of the human prolactin gene by cAMP requires two cis-acting elements and at least the pituitary-specific factor Pit-1.
The Journal of biological chemistry - 25 Sept 1991
Peers B, Monget P, Nalda M A, Voz M L, Berwaer M, Belayew A, Martial J A
Abstract excerpt
To identify the cis-acting elements responsible for cAMP stimulation of human prolactin (hPRL) promoter activity, pituitary GC cells were transfected with 5'-deleted hPRL promoters fused to the chloramphenicol acetyltransferase reporter gene. The proximal regulatory region (coordinates -250 to -4...
Topics
- Animals
- Base Sequence
- Binding Sites
- Blotting, Southern
- Blotting, Western
- Cattle
- Chloramphenicol O-Acetyltransferase
- Cyclic AMP
- DNA-Binding Proteins
- Humans
- Molecular Sequence Data
- Mutation
- Plasmids
- Prolactin
- Promoter Regions, Genetic
- Rats
- Sequence Homology, Nucleic Acid
- Thymidine Kinase