The pSC101 par locus alters protein-DNA interactions in vivo at the plasmid replication origin.

doi:10.1128/jb.175.18.6046-6048.1993

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Article

The pSC101 par locus alters protein-DNA interactions in vivo at the plasmid replication origin.

Journal of bacteriology - 1 Sept 1993

Ingmer H, Cohen S N

Abstract excerpt

We report here direct evidence that mutations in the par locus affect protein-DNA interactions in vivo at the replication origin of plasmid pSC101. Concomitant with par-mediated plasmid stabilization, two sites in the origin region show an altered methylation pattern as detected by in vivo footpr...

Topics

Base Sequence
DNA Replication
DNA, Bacterial
DNA-Binding Proteins
Densitometry
Escherichia coli
Methylation
Molecular Sequence Data
Mutation
Plasmids
Repetitive Sequences, Nucleic Acid

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