Article
Roles of DNA polymerase I in leading and lagging-strand replication defined by a high-resolution mutation footprint of ColE1 plasmid replication.
Nucleic acids research - 1 Sept 2011
Allen Jennifer M, Simcha David M, Ericson Nolan G, Alexander David L, Marquette Jacob T, Van Biber Benjamin P, Troll Chris J, Karchin Rachel, Bielas Jason H, Loeb Lawrence A, Camps Manel
Abstract excerpt
DNA polymerase I (pol I) processes RNA primers during lagging-strand synthesis and fills small gaps during DNA repair reactions. However, it is unclear how pol I and pol III work together during replication and repair or how extensive pol I processing of Okazaki fragments is in vivo. Here, we address these questions by analyzing pol I mutations generated through error-prone replication of ColE1 plasmids. The data...
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