Article
The mutagenic footprint of low-fidelity Pol I ColE1 plasmid replication in E. coli reveals an extensive interplay between Pol I and Pol III.
Current genetics - 1 Aug 2014
Troll Christopher, Yoder Jordan, Alexander David, Hernández Jaime, Loh Yueling, Camps Manel
Abstract excerpt
ColE1 plasmid replication is unidirectional and requires two DNA polymerases: DNA polymerase I (Pol I) and DNA polymerase III (Pol III). Pol I initiates leading-strand synthesis by extending an RNA primer, allowing the Pol III holoenzyme to assemble and finish replication of both strands. The goal of the present work is to study the interplay between Pol I and Pol III during ColE1 plasmid replication, to gain new...
Topics
- DNA Polymerase I
- DNA Polymerase III
- DNA Repair
- DNA Replication
- Escherichia coli
- Gene Order
- Mutation
- Plasmids
- Protein Binding
- Replication Origin