Increased mitogenic responsiveness of Swiss 3T3 cells expressing constitutively active Gs alpha.

doi:10.1016/0006-291x(90)91154-k

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Article

Increased mitogenic responsiveness of Swiss 3T3 cells expressing constitutively active Gs alpha.

Biochemical and biophysical research communications - 16 May 1990

Zachary I, Masters S B, Bourne H R

Abstract excerpt

Mutational replacement of glutamine-227 with a leucine residue in the GTP-binding domain of the alpha subunit of GS (Q227L alpha S) reduces its ability to hydrolyse GTP and causes constitutive activation of the mutant protein. Expression in Swiss 3T3 fibroblasts of Q227L alpha S caused markedly increased basal adenylyl cyclase activity, enhanced intracellular cyclic AMP (cAMP) accumulation and increased mitogenic...

Topics

Adenylyl Cyclases
Amino Acid Sequence
Animals
Cell Line
Cell Membrane
Cell Transformation, Neoplastic
Cyclic AMP
Enzyme Activation
GTP-Binding Proteins
Glutamine
Guanosine 5'-O-(3-Thiotriphosphate)

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Guanosine Triphosphate

Hydrolysis

Immunoblotting

Leucine

Mitogens

Molecular Sequence Data

Mutation

Source

Open in PubMed

Identifiers

PMID: 2161217
DOI: 10.1016/0006-291x(90)91154-k

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2161217-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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