Article
Regulation of cAMP-mediated gene transcription by wild type and mutated G-protein alpha subunits. Inhibition of adenylyl cyclase activity by muscarinic receptor-activated and constitutively activated G(o) alpha.
The Journal of biological chemistry - 18 Nov 1994
Migeon J C, Thomas S L, Nathanson N M
Abstract excerpt
We have used a luciferase reporter gene under the transcriptional control of a cAMP response element (CRE) to monitor the effects of G-protein alpha subunits on cAMP-regulated gene expression and to examine muscarinic acetylcholine receptor (mAChR) functional coupling to G-proteins. Expression in...
Topics
- Adenylyl Cyclase Inhibitors
- Animals
- Cell Line
- Colforsin
- Cyclic AMP
- GTP-Binding Proteins
- Gene Expression Regulation, Enzymologic
- Luciferases
- Mutation
- Myristic Acid
- Myristic Acids
- Rats
- Receptors, Muscarinic
- Transcription, Genetic
- Transfection