Article
Transcriptional effects in GH3 cells of Gs alpha mutants associated with human pituitary tumors: stimulation of adenosine 3',5'-monophosphate response element-binding protein-mediated transcription and of prolactin and growth hormone promoter activity via protein kinase A.
Endocrinology - 1 Oct 1995
Gaiddon C, Mercken L, Bancroft C, Loeffler J P
Abstract excerpt
Somatic mutations of the alpha-subunit of Gs (Gs alpha) have been detected previously at high frequency in human PRL- and/or GH-producing pituitary tumors. To test whether these mutants are responsible for the increased production of these hormones, we used transient cotransfection assays to analyze their genomic effects in GH3 rat pituitary cells. We first show that guanosine triphosphatase (GTPase)-deficient Gs...
Topics
- Cyclic AMP Response Element-Binding Protein
- Cyclic AMP-Dependent Protein Kinases
- GTP-Binding Proteins
- Growth Hormone
- Humans
- Mutation
- Pituitary Neoplasms
- Prolactin
- Promoter Regions, Genetic