Article
GH3 cells expressing constitutively active Gs alpha (Q227L) show enhanced hormone secretion and proliferation.
Molecular and cellular endocrinology - 14 Mar 1997
Ham J, Ivan M, Wynford-Thomas D, Scanlon M F
Abstract excerpt
cAMP levels in GH3gsp cells (Q227L mutation of Gs alpha), in comparison with uninfected GH3 and GH3vt (with vector alone) cells, were two to three fold (P< 0.01) higher (basal), and 10-20 fold (P<0.001) higher (in the presence of isobutyl methylxanthine, (IBMX)). Proliferation of GH3gsp cells aft...
Topics
- 1-Methyl-3-isobutylxanthine
- Blotting, Western
- Bucladesine
- Cell Count
- Cell Division
- Colforsin
- Cyclic AMP
- Enzyme Activation
- GTP-Binding Proteins
- Growth Hormone
- Humans
- Mutation
- Prolactin
- Thymidine
- Transfection
- Tumor Cells, Cultured