Mutation of the serine 312 phosphorylation site does not alter the ability of mouse p53 to inhibit simian virus 40 DNA replication in vivo.

Abstract excerpt

Two mutations were introduced into the wild-type mouse p53 gene by oligonucleotide-directed mutagenesis. These mutations substituted alanine or aspartic acid for serine at position 312, which is constitutively phosphorylated. Phosphopeptide mapping of the mutant proteins, expressed in COS cells, confirmed the loss of phosphorylation at position 312. There were no changes in the ability of the mutant p53s to...

Topics

• Animals
• Base Sequence
• Cell Line
• DNA Replication
• DNA, Viral
• Mice
• Molecular Sequence Data
• Mutation
• Nuclear Proteins
• Oncogene Proteins
• Phosphoproteins
• Phosphorylation