Article
Phosphorylation studies on rat p53 using the baculovirus expression system. Manipulation of the phosphorylation state with okadaic acid and influence on DNA binding.
European journal of biochemistry - 15 Mar 1995
Fuchs B, Hecker D, Scheidtmann K H
Abstract excerpt
To elucidate the role of phosphorylation of p53 we used the baculovirus expression system to obtain high yields of protein eventually in distinct phosphorylation states. Initially, we obtained only marginal phosphorylation, despite high levels of expression. Two-dimensional phosphopeptide maps ex...
Topics
- Amino Acid Sequence
- Animals
- Antigens, Polyomavirus Transforming
- Baculoviridae
- Base Sequence
- Cloning, Molecular
- Cyclin-Dependent Kinases
- DNA
- DNA-Binding Proteins
- Ethers, Cyclic
- Molecular Sequence Data
- Mutation
- Okadaic Acid
- Peptide Mapping
- Phosphoprotein Phosphatases
- Phosphorylation
- Protein Conformation
- Rats