Article
M153R mutation in a pH-sensitive green fluorescent protein stabilizes its fusion proteins.
PloS one - 3 May 2011
Morimoto Yusuke V, Kojima Seiji, Namba Keiichi, Minamino Tohru
Abstract excerpt
BACKGROUND: Green fluorescent protein (GFP) and its fusion proteins have been used extensively to monitor and analyze a wide range of biological processes. However, proteolytic cleavage often removes GFP from its fusion proteins, not only causing a poor signal-to-noise ratio of the fluorescent images but also leading to wrong interpretations. METHODOLOGY/PRINCIPAL FINDINGS: Here, we report that the M153R mutation...
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