Article
Structural basis of fluorescence fluctuation dynamics of green fluorescent proteins in acidic environments.
The journal of physical chemistry. B - 30 Nov 2006
Liu Yuexin, Kim Hye-Ryong, Heikal Ahmed A
Abstract excerpt
Green fluorescent proteins (GFPs) have become powerful markers for numerous biological studies due to their robust fluorescence properties, site-specific labeling, pH sensitivity, and mutations for multiple-site labeling. Fluorescence correlation spectroscopy (FCS) studies have indicated that fluorescence blinking of anionic GFP mutants takes place on a time scale of 45-300 ms, depending on pH, and have been...
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