Conditional poliovirus mutants made by random deletion mutagenesis of infectious cDNA.

doi:10.1128/JVI.64.1.185-194.1990

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Article

Conditional poliovirus mutants made by random deletion mutagenesis of infectious cDNA.

Journal of virology - 1 Jan 1990

Kirkegaard K, Nelsen B

Abstract excerpt

Small deletions were introduced into DNA plasmids bearing cDNA copies of Mahoney type 1 poliovirus RNA. The procedure used was similar to that of P. Hearing and T. Shenk (J. Mol. Biol. 167:809-822, 1983), with modifications designed to introduce only one lesion randomly into each DNA molecule. Methods to map small deletions in either large DNA or RNA molecules were employed. Two poliovirus mutants, VP1-101 and...

Topics

Amino Acid Sequence
Animals
Base Sequence
Capsid
Cell Line
Chromosome Deletion
DNA, Viral
Genes, Viral
HeLa Cells
Humans
Molecular Sequence Data
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Nucleotide Mapping

Oligonucleotide Probes

Phenotype

Plasmids

Poliovirus

RNA, Viral

Source

Open in PubMed Open full text

Identifiers

PMID: 2152811
DOI: 10.1128/JVI.64.1.185-194.1990
PMCID: PMC249081

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2152811-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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