A site-directed mutagenesis method utilising large double-stranded DNA templates for the simultaneous introduction of multiple changes and sequential multiple rounds of mutation: Application to the study of whole viral genomes.

doi:10.1016/j.jviromet.2006.05.034

Article

A site-directed mutagenesis method utilising large double-stranded DNA templates for the simultaneous introduction of multiple changes and sequential multiple rounds of mutation: Application to the study of whole viral genomes.

Journal of virological methods - 1 Oct 2006

Liu Li, Lomonossoff George

Abstract excerpt

A new technique for conducting site-directed mutagenesis was developed. This method allows the colour selection of mutants through the simultaneous activation or deactivation of the alpha-peptide of beta-galactosidase. Double-stranded DNA plasmids containing large inserts (at least 6.4 kbp in the present experiments) can be used as the mutational template. The method can efficiently create mutations at multiple...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

A site-directed mutagenesis method utilising large double-stranded DNA templates for the simultaneous introduction of multiple changes and sequential multiple rounds of mutation: Application to the study of whole viral genomes.

Abstract excerpt

Topics

Related research