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Article

Quantitative aspects of the mutant analysis by PCR and restriction enzyme cleavage (MAPREC).

PCR methods and applications - 1 Dec 1993

Lu Z, Douthitt M P, Taffs R E, Ran Y, Norwood L P, Chumakov K M

Abstract excerpt

Quantitation of virus revertants by PCR and restriction enzyme cleavage may give nonlinear results and, in some cases, produce artifacts caused by nucleotide misincorporation and heteroduplex formation, occurring during PCR. Modifications of the procedure allowed us to overcome these problems and develop a highly sensitive and reliable method of mutant quantitation. This procedure can be used to assess the...

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PMID: 8118399
DOI: 10.1101/gr.3.3.176

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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