Article
Quantitative aspects of the mutant analysis by PCR and restriction enzyme cleavage (MAPREC).
PCR methods and applications - 1 Dec 1993
Lu Z, Douthitt M P, Taffs R E, Ran Y, Norwood L P, Chumakov K M
Abstract excerpt
Quantitation of virus revertants by PCR and restriction enzyme cleavage may give nonlinear results and, in some cases, produce artifacts caused by nucleotide misincorporation and heteroduplex formation, occurring during PCR. Modifications of the procedure allowed us to overcome these problems and...
Topics
- Base Sequence
- DNA Mutational Analysis
- DNA Primers
- DNA Restriction Enzymes
- Hydrolysis
- Molecular Sequence Data
- Mutation
- Nucleic Acid Heteroduplexes
- Poliovirus
- Poliovirus Vaccine, Oral
- Polymerase Chain Reaction